7.  Kevin Ahern’s Biochemistry – Protein Purification II

7. Kevin Ahern’s Biochemistry – Protein Purification II

Professor Kevin Ahern: Happy Monday! Monday, Monday, I have a song I’m writing called Hyundai, Hyundai, I’m sure you can figure out the tune it goes to. Hope everybody had a good weekend. Anyone remember it? Alright, enough silliness. We spent some time last time talking about techniques and today I’m going to spend some …

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#07 Biochemistry Protein Characterization Lecture for Kevin Ahern’s BB 450/550

#07 Biochemistry Protein Characterization Lecture for Kevin Ahern’s BB 450/550

Another Monday beckons, another week beckons. One day closer to an exam. Student: Whoo! Kevin Ahern: Yay, huh? One day closer to your opportunity to show me how much you know. That’s good. I hope you had a good weekend. Student: Fantastic. Kevin Ahern: Fantastic? Student: We won. Kevin Ahern: Are we talking about football …

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Milk or BSA? Choosing a blocking protein for Western Blotting (WB) | CST Tech Tips

Milk or BSA? Choosing a blocking protein for Western Blotting (WB) | CST Tech Tips

What protein should I use to block a transfer membrane for a western blot? My name is Srikanth, I’m a product scientist at Cell Signaling Technology, and this is CST Tech Tips. (music chiming) In regards to western blotting, a common question we always get is, what should I use, milk or BSA to block? …

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Separate proteins by SDS-PAGE for Western blot

Separate proteins by SDS-PAGE for Western blot

The first step in many protein analytical methods, including Western blot analysis, is to separate the proteins in a sample by gel electrophoresis. The most widely used protein electrophoretic technique is sodium dodecyl sulfate polyacrylamide gel electrophoresis, or SDS-PAGE, in which proteins are denatured and evenly charged by the ionic detergent SDS so that the …

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Separating Proteins using SDS Polyacrylamide Gel Electrophoresis

Separating Proteins using SDS Polyacrylamide Gel Electrophoresis

Edvotek Instructional Video: Separating Proteins Using SDS Polyacrylamide Gel Electrophoresis (also known as SDS-PAGE) For this experiment, you will need: • Protein samples • Tris-glycine-SDS electrophoresis buffer • A vertical polyacrylamide gel electrophoresis apparatus • A micropipette and gel loading tips • A power supply • And, a precast polyacrylamide gel STEP 1. BOIL the …

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