How to Use the New ChemiDoc MP for Protein Gel Imaging

How to Use the New ChemiDoc MP for Protein Gel Imaging


In this video you’ll learn how to log
in to your ChemiDoc, use smart tray technology, capture protein gel images in
three easy steps, how do you stain-free imaging and export your images To turn your ChemiDoc on push the green button in front of your instrument When the customizable home screen appears tap anywhere to bring up the log in menu If you’re a new user type the desired username. Click the plus sign and the new
log in will be entered. Administrative controls can be set by clicking the person icon in the top right hand corner To begin, open the imager and pull out
the transilluminator Remember that all gels and blots are always imaged on one
of three trays. It is easy but important to clean the trays Simply use DI water
and a kimwipe for the best performance Smart tray technology automatically
recognizes application-specific trays and adjusts imaging parameters and
software options accordingly For colorimetric stains select the white tray Center your sample on the tray When you are satisfied with the position push the transilluminator back in and close the drawer Zoom into the desired imaging area If you prefer, simply select small, medium, or large under image size Tap application
to select the appropriate category and the stain for your sample In this example we are examining a protein gel stained with our Bio-Safe Coomassie Stain You can take a preview of your image and set your region of interest This can be used for excluding very bright areas that you won’t want to
quantify such as your marker Choose between entering your exposure time or enabling automatic detection of your bands Tap the camera button and capture
the image Bio-Rad Stain-Free gels are an excellent alternative to Coomassie Blue no staining or destaining is required just a simple 45 second UV activation
step After you’ve completed electrophoresis place the Stain-Free Gel directly on the UV tray when you are satisfied with the position push the transilluminator back in and close the drawer Select Stain-Free Gel as your
application then under exposure select a gel activation time of 45 seconds for
blotting or use 5 minutes for increased sensitivity which can be helpful if the stained gel is the final product Tap the camera button and capture the image If blotting stain free gels can be visualized on the membrane and serves as both an effective transfer efficiency check and total protein stain For a rapid look at the band intensities you can tap each band and the relative
fluorescence units as well as percent to saturation will appear To adjust your image, tap the transform icon in the lower toolbar Adjustments are automatically saved but the actual data will remain unaffected Your images will be automatically saved
and exported to the gallery Tap the image info icon Here you can enter a
custom name and any notes you’d like to include about your image or experiment Tap the Send/Save icon to select the
file format you wish to export You can export images in multiple file types Under export options, tap a file type and the destination either saving to a
USB Drive or to a Network If you choose the Image Lab file format the custom name notes and all the data included below are exported to Bio-Rad’s license
free Image Lab analysis software available for both Mac and PC We hope that this instructional video has showed you how to capture a protein gel image For further learning please utilize the complete searchable on-board manual as well as the teach as you tap software assistance tool

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