Comments

(7 Comments)

  • Salvador Hirth

    Thank you Professor Britt Glaunsinger, I didn't know much about the herpes virus and you explain everything clearly! Do you know if a restriction enzyme specific for +ssRNA that cleaves near the palindromic sequences CAAGCUUG or AAGCUU, has been already isolated? I believe it should look similar to the restriction enzyme Hind III. Another question: do you think that it is possible to use fusion peptides from a virus, to make a restriction enzyme cross the cell membrane so that the enzymes could be delivered into the cytoplasm to cleave viral genomes?

  • Salvador Hirth

    So, SOX is a nuclease encoded by the HV, it must be degraded sometime, I wonder how long SOX remains in the cytoplasm and whether or not some accidentaal nucleus signal could allow it into the cell nucleus. If SOX get into the nucleus, recently assembled mRNA would be cleaved even before reaching the cytoplasm.

  • Salvador Hirth

    This is one of the most interesting lectures I've ever watched, you're amazing, Prof. Glausinger! I'm going to watch your explanations sometimes; at first I had the impression that HV infection could somehow at least for a short time, cleave also part of the mRNA from other viruses.

  • Salvador Hirth

    How did you suppress Xrn1, did you use a restriction enzyme to remove the gene for Xrn1?

  • Salvador Hirth

    I hope that the long Coronavirus genome lack any Nuclease escape elements, those would protect the Coronavirus messenger RNA against cleavage by multiple viral endonucleases and my idea to use a specific restriction enzyme to fight the infection would be useless.

  • Salvador Hirth

    Gosh, this is a plethora of information!

  • ShakespeareCafe

    Grabs a seat in the front row for YouTube University’s Department of Cellular and Molecular Biology Virology 101

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